[Abstract] 

Molecular signatures that make possible to understand diseases has been relatively insufficient. Mass spectrometry-based Omics technologies such as proteomics and metabolomics are widely applied for identification and characterization of new molecular signatures. Proteomics for secretome represents a powerful strategy for characterizing and quantifying proteins secreted by a given cell under specific conditions. Secretory proteins play important roles in the cross-talk of individual functional units, including cells. Since secretory proteins are essential for signal transduction, they are closely related with disease development, including metabolic and neural diseases. In neural diseases, neurons, astrocytes and microglia cells secrete cytokines and chemokines that play roles in neurotoxic and neuroprotective responses. We have been found new regulators from metabolic disease, liver cancer and Alzheimer’s disease using the mass spectrometry-based Omics approaches. We will introduce those results with recent technological advances.