An internalizing antibody targeting of cell surface GRP94 effectively suppresses tumor angiogenesis of colorectal cancer
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open access
Graphical Abstract
Abbreviations
CRC
colorectal cancer
GRP94
glucose-regulated protein 94
MVD
microvessel density
mAb
monoclonal antibody
5-FU
5-fluorouracil
FOLFIRI
leucovorin, 5-FU and irinotecan
FOLFOX
Leucovorin, 5-FU, and oxaliplatin
FDA
Food and Drug Administration
EGF
epidermal growth factor
VEGF
vascular endothelial growth factor
ER
endoplasmic reticulum
TMA
tissue microarray
IHC
immunohistochemistry
HUVECs
human umbilical vein endothelial cells
FBS
fetal bovine serum
rhGRP94
recombinant human GRP94
PCR
polymerase chain reaction
scFv
single-chain variable fragment
ELISA
enzyme-linked immunosorbent assay
CDR
complementarity-determining region
IgG
immunoglobulin G
BSA
bovine serum albumin
PBS
phosphate-buffered saline
PBS-T
PBS containing 0.05% (v/v) Tween 20
HRP
horseradish peroxide
TMB
3,3′,5,5′-tetramethylbenzidine
SPR
surface plasmon resonance
PFA
paraformaldehyde
hTNFα
human tumor necrosis factor α
ICAM-1
intercellular cell adhesion molecule-1
VCAM-1
vascular cell adhesion molecule-1
TBS-T
Tris buffered saline with 0.1% (v/v) Tween 20
ERK
extracellular signal-regulated kinase
OCT
optimal cutting temperature
TUNEL
terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling
DAPI
4′,6-diamidino-2-phenylindole, dihydrochloride
GOT
glutamic oxaloacetic transaminase
GPT
glutamic pyruvic transaminase
TBIL
total bilirubin
CRE
creatinine
BUN
blood urea nitrogen
ANOVA
analysis of variance
SEM
standard error of the mean
Keywords
Cell surface GRP94
Tumor angiogenesis
Fully human antibody
Internalization
Downregulation
Cited by (0)
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These authors contributed equally to this work.
© 2022 The Author(s). Published by Elsevier Masson SAS.